Development and validation of analytical method for the estimation of lamivudine in rabbit plasma / 药物分析学报

Lamivudine has been widely used in the treatment of HIV disease. A reliable, sensitive reversed phase high performance liquid chromatography (RP-HPLC) method was developed and validated for lamivudine in rabbit plasma. The method was developed on Hypersil BDS C-18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase of 0.25％ Triethylamine buffer (pH 3.0):acetonitrile (70:30, v/v). The efficient was monitored by UV detector at 256 nm. The total run time was 15 min with a flow rate of 1.0 mL/min. Calibration curve was linear over the concentration range of 25-2000 ng/mL. The retention times of lamivudine and internal standard (Nelfinavir) were 8.78 min and 10.86 min, respectively. The developed RP-HPLC method can be successfully applied for the quantitative pharmacokinetic parameters determination of lamivudine in rabbit model.

Geriatric Mental Health in a Tertiary Care Centre: Socio-Demographic and Clinical variables.

Background: Nearly 50% of the aged have some physical ailment and are in need of active health care. Geriatric psychiatry is a sub-specialty of psychiatry, which deals with psychopathology occurring in the elderly and the various aetiological factors which cause the mental disorders in old age. Method: This is a descriptive study with sampling in a tertiary centre where the diagnoses were made based on the tenth edition of the International Statistical Classification of Diseases and Related Health Problems (ICD- 10) and were evaluated for sociodemographic and clinical variables as well as general medical conditions. Results: Mean age of the patients was 69.81 years, male constituted 73.58% and female 26.42%, Hindu were 56.60% and Islam 43.40%. Mean duration of stay in psychiatry ward was 5.7 days. 77.36% of patients were discharged on advice, 15.09% were transferred to another department, 1.89% absconded, 1.89% referred to another hospital and 3.77% referred to another department. 9.62% patients had general medical conditions alone and 40.38% had psychiatric disorders alone while 50% had combined psychiatric disorders with general medical conditions. 42.31% patients had organic, including symptomatic, mental disorders; 3.85% had mental and behavioural disorders due to psychoactive substance use; 26.92% had schizophrenia, schizotypal and delusional disorders; 28.85% had mood [affective] disorders; 5.77% patients had neurotic, stress-related and somatoform disorders. Conclusion: Within the limitations of nongeneralizability of findings, present study throws light in important areas of geriatric mental health including general medical conditions.

Coronary Artery Dilatation in Incomplete Kawasaki Disease.

We conducted this study to compare the incidence of coronary artery dilatation in children with Incomplete and Classical Kawasaki disease, diagnosed as per AHA criteria. Subjects were included on a retrospective review of records (2002-2007); those with a discharge diagnosis of Kawasaki disease were enrolled. A total of 29 patients were identified (3.1 per 1000 pediatric admissions), out of which 22 were boys (median age: 4.8 years; range: 4 months-11 years). Seventeen (58.6%) had Classical KD and twelve (41.4%) children had Incomplete KD. All children received IVIG and underwent echocardiography. Coronary involvement was more in Incomplete KD (11/12 = 91.6 %) as compared to Classical KD (7/17= 41.1%). The sensitivity, specificity and predictive value of AHA criteria to detect coronary artery dilatation was 39%, 9%, and 41%, respectively. We conclude that children presenting with Incomplete Kawasaki disease are at a higher risk of coronary artery abnormalities.